Laser 2000 (UK): Inverted Epifluorescence Microscope
Published on Nov. 08, 2016
Customer Application
Laser 2000 integrated Zaber's ASR series XY microscope stage with an inverted epifluorescence microscope to enable automated scanning of two samples. Both samples are epithelial cells from a human embryonic kidney. Multiple fluorescent stains were employed to allow for bright and high-contrast images that depict the internal structure of the cells. One such stain was DAPI, which is a fluorescent dye that intercalates with DNA. Different stains bond to specific types of biomolecules, such as those found in microtubules, actin cytoskeleton, or endoplasmic reticulum. In order to excite multiple fluorescent stains at precise intervals, Zaber's X-FWR motorized filter wheel was mounted between the microscope lamp housing and the excitation port. The filter wheel can change to an adjacent optic at high speed, taking only 57 ms between exposure times. By combining Zaber's filter wheel with their XY stage and controlling both via standard µManager software, fluorescence channels were switched at high rates, resulting in short multi-channel capture times and high throughput.
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 Close-up of Zaber's motorized filter wheel mounted between the microscope lamp housing and the excitation port. |
About Laser 2000 (UK)
Laser 2000 supplies state-of-the-art photonic and optoelectronic products. Their range includes motion control, optical filters, LED light sources, lasers, laser safety, cameras, adaptive optics, and much more. With over 25 years of experience in the market, they help to find the best possible solution to your application.
Laser 2000 (UK) exclusively distributes Zaber products to the UK and Ireland. For general enquires or a quotation, please contact them by phone +44 (0) 1933 461 666, email sales@laser2000.co.uk, or visit the Laser 2000 website at https://www.laser2000.co.uk/.